Dr. Magdaline Joseph, Emmanuel Oluwamuyiwa Oje, Ameh Agbo Adams,Hajara lakka Durkwa, Jubilee Dauda, Abalaka Nathan Eneojo, Chioma Favour Onyeka

University of Ibadan; Email: magdalinejoseph8@gmail.com

Corresponding Author: Dr. Magdaline Joseph, University of Ibadan; Email: magdalinejoseph8@gmail.com

Citation: Dr. Magdaline Joseph (2025) A Metagenomic Approach to Microbiota Occurrence in Mixed Waste and Their Methanogenic, Lignolytic and Indutrial Pottensials. World J Multidiscip Res Rev, 3(1);1-6.

Copyright: © 2025, Dr. Magdaline Joseph. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.

ABSTRACT

This work demonstrated the requisite necessity of microorganisms derived from waste feed-stock in environmental, industrial and metabolic processes by confirming their degradative and gas producing capacity. Methanogens and other logninolytic microorganisms were isolated and identified. This study aimed at isolation and identification using molecular characterization and metagenomic approach from waste derived microbiome and their potentials. The organisms of interest are the ligninolytic and gas producing microbes (methanogens). Samples (Fruit and vegetable residuez+wood waste+animal waste) were collected from AMAC Relocation market, Lugbe, F.C.T. and analysed at the International Institute for Tropical Agriculture ((IITA) Oyo state, the 16SrNA and mcrA encoding the alpha ά unit of methyl co-enzyme reductase genes as markers was used for the detection of phylogenic analysis of anaerobic bacteria community, a metagenomic approach was also carried out for identification. Mineral solution containing CuSO₄ was used to identify cellulose degraders. Their degradative ability was confirmed by the formation of a clear zone. Twenty-four anaeroboic bacteria were isolated out of which six bacteria species were identified as Methanothermobacter thermautotrophicus NPK, Candidatus methanoperedenaceae GB37, Methanothermobacter thermautotrophicus CCSD, Methanobacteriaceae archaeon KR-H07 A06, Methanobacteriaceae archaeon RK-H07, and Candidatus methanoperedenaceae GB50. Biogas yield increased from 3.0 to 8.0mL in the non-inoculated sample while the inoculated ranged from 5.0 - 24.0mL on day 30 with higher gas yield in the inoculated compared to the non-inoculated sample, gas yield increased from 0mL - 5mL on day 60 in the inoculated as compared to the non-inoculated. All the methanogens identified produce gas in their metabolic processes of breaking down the complex polymeric substances from the waste derived feedstock to smaller monomeric units. A total of 180 bacterial species were found in the non-inoculated sample of which Bacteroidota (26.91%), Fermicutes (25.87%) and Proteobacteria (7.24%) predominated. Seventy (70.0%) in the non-inoculated were anaerobes while 30% were aerobes. Clostridium were the highest having (28.8%) amongst the anaerobic bacterial community while Acidobacteriota was the least (0.01%). Eschericia coli was the highest aerobic species having (14.5%) while Streptomyces was the least (0.01%). The ability to produce ligninolytic enzyme makes some microorganisms suitable for application in many industries, including the production of biofuels, antibiotics, bioremediation, biomedical applications as biosensors.

Keywords: Mixed-waste, Methanogens, Metagenomic analysis, Biogas, Molecular characterization, and Potentials.